Chromatographic patterns of protein and enzymes in extracts of rhabdomyosarcoma and muscle in mice.

Soluble proteins of rhabdomyosarcoma and normal muscle were chromatographed and separated into a number of peaks by gradient elution from DEAE cellulose. The chromatographic patterns were different in the two tissues studied. Various enzymatic activities were determined in the fractions and localized in the chromatograms of both muscle and rhabdomyosarcoma. Some of the enzymes tested appeared in multiple peaks and showed a pattern of distribution in tumor tissue different from that in the muscle.